If you burn a previous dumped ISO to a DVD±R/RW disc then use File => Boot from DVD backup in Dolphin, the game will work fine, however, Dolphin will be a stuttery mess when data is being streamed from the disc because AFAICT the code responsible for this "feature" hasn't been touched for ages. It currently uses hard-coded values for things like disc seek time that doesn't match the actual seek time from your DVD drive, which generally takes longer. When this happens, Dolphin will stall the emulation until the drive is ready to stream the data, which in turn will produce stutters.
Another particular issue is that different from the GC/Wii disc drive, PC DVD drives will stop spinning after a period of inactivity and Dolphin currently doesn't do anything to prevent that. If the game needs to read something while the DVD drive isn't spinning, you'll get a freeze of around 3 to 5 seconds, which is the time it generally takes for the drive to start spinning the disc again and read the requested data.
TL;DR you can boot DVD backups in Dolphin but the overall experience is generally poor...
Another particular issue is that different from the GC/Wii disc drive, PC DVD drives will stop spinning after a period of inactivity and Dolphin currently doesn't do anything to prevent that. If the game needs to read something while the DVD drive isn't spinning, you'll get a freeze of around 3 to 5 seconds, which is the time it generally takes for the drive to start spinning the disc again and read the requested data.
TL;DR you can boot DVD backups in Dolphin but the overall experience is generally poor...
Avell A70 MOB: Core i7-11800H, GeForce RTX 3060, 16 GB DDR4-3200, Windows 11 (Insider Preview)
ASRock Z97M OC Formula: Pentium G3258, GeForce GT 440, 16 GB DDR3-1600, Windows 10 (22H2)
ASRock Z97M OC Formula: Pentium G3258, GeForce GT 440, 16 GB DDR3-1600, Windows 10 (22H2)